Information-enriched characterization of mobile populations. Morphological facts from illustrations or photos can insert on the richness of flow cytometry details. As an example, the figure demonstrates an otherwise regular apoptosis assay using Annexin V and PI, including mobile imaging to characterize cells in Each and every populace to reveal morphologically unique functions. These insights could not are already received from multiplex staining alone.

No matter if you’re a beginner or an expert, examine our methods to advance your exploration and experience in flow cytometry.

(B) RAW 264.seven macrophages are sometimes annotated inaccurately with regard to particle rely because of their complexity and noncircular borders. The amount of singlets accessible for analysis improves once the consumer trains and employs a far more accurate model.

Uncover new insights into your experimental information by visualizing populations employing merged fluorescence and morphology data. Our software package interface wizards empower downsampling, concatenation (merged information) and dimensionality tools which have been crafted in the application for an intuitive, streamlined knowledge Investigation working experience.

"A picture isn't really pretty really worth a thousand dots, but it definitely helps to see images. It is great to see visuals that correlate with staining. As an example, AnnexinV-optimistic cells which can be morphologically various than nutritious cells, or CD14+ monocytes which are much larger and more textured than CD3+/CD4+/CD14- lymphocytes.

Below, a highly skilled user has gated singlets confidently. Right after assessing the guide singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate incorporates greater than four% aggregates.

Optimizing gating technique. Even strong guide singlet gating is error-prone and remains a subjective final decision place in Just about all flow cytometry assays. Imaging can be used to verify and adjust gates to include only single cells of curiosity.

Constant picture high quality even at high throughput Acoustic concentrating along with a large-speed digital camera Mix to picture these T cells continuously at lower or higher flow costs. Conveniently change concentrate and digicam options to meet experimental demands.

The Attune NxT cytometer will be the reliable workhorse instrument your lab can count on for consistent and dependable general performance. The Attune CytPix product contains a high-velocity brightfield camera and AI-driven impression Investigation application, with current upgrades including equipment Discovering tools and an intuitive user interface, enabling Improved facts precision, precision, and quicker insights into sample biology.

Potentially most significantly, these gatherings incorporate cells of Evidently diverse phenotypes which could lead to incorrect conclusions about double constructive gatherings (especially in scarce populations).

AI-driven automated impression Evaluation with the Attune Cytometric Application interprets function attributes into unique morphology parameters that may be combined with typical fluorescence and scatter parameters to reveal very reproducible inhabitants adjustments and exceptional subsets.

Application Be aware: Automated image Assessment reduces consumer-to-user variability in flow cytometry gating procedures

Created-in dimensionality reduction equipment increased resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to verify expected populace resolution in lysed total human blood. User can check out events from Every single cluster for visual confirmation of mobile phenotypes.

No wash/no lyse leukocyte Investigation with imaging. Cells have been obtained from 24-hour-old blood by dilution in 1 mM EDTA ( Skilled UX/UI enables buyers to prepare AI types for expanded purposes. (A) The “Educate Product” function delivers annotation resources and genuine-time product schooling accuracy.

Gating on these extended parameters enables you to speedily and properly discover populations of desire and ensure gating method with little or no handbook evaluation. The Attune software program presents usage click here of state-of-the-art machine Understanding Evaluation tools inside of an expertly designed consumer interface, which makes it exceptionally user friendly.

The Attune Xenith cytometer is often a substantial-parameter spectral analyzer, giving each spectral unmixing and traditional flow abilities for comprehensive and versatile mobile Assessment.

The Attune CytPix Flow Cytometer enables buyers to visualise samples in the course of acquisition, confirm the precision of gating tactics, and assist final results with dependable documentation for publication applications.

In this article, we display that by gating strategies using graphic-based mostly quantitative parameters can distinguish interacting cells from coincident functions to additional properly evaluate interacting cells.